The production of Genetically modified organisms is a multistep process. The first stage in producing GMO is the identification and isolation of the gene of interest. The second stage is the insertion of DNA into the organism which is done by different methods.
Methods of GMO production:
There are two common methods which are used for GMO production.
Particle/Gene Gun Method:
In this method, metal particles of the gun are coated with our desired DNA. Then the DNA is injected into the organism by bombardment of particles. Read more
Agrobacterium Tumefaciens Method:
Different bacterial types and viruses are used to transfer DNA into the cells of organisms as a part of their life cycle. However, the production of GMO is usually done by using bacteria called agrobacterium tumefaciens. The gene of bacterium containing DNA is transferred into the cells of the bacterium which is then transferred into the host plant cells. The cells of the host plant accept the new DNA and then produce new plants. This is possible because each single cell of plant can generate a new full plant. Read more
On rare occasions, the DNA transfer process takes place naturally. One example is sweet potatoes that contains the DNA sequence which was transferred by agrobacterium tumefaciens into their genome thousands of years ago.
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